A Link between Integral Membrane Protein Expression and Simulated Integration Efficiency.

ABSTRACT: Integral membrane proteins (IMPs) control the flow of information and nutrients across cell membranes, yet IMP mechanistic studies are hindered by difficulties in expression. We investigate this issue by addressing the connection between IMP sequence and observed expression levels. For homologs of the IMP TatC, observed expression levels vary widely and are affected by small changes in protein sequence. The effect of sequence changes on experimentally observed expression levels strongly correlates with the simulated integration efficiency obtained from coarse-grained modeling, which is directly confirmed using an in vivo assay. Furthermore, mutations that improve the simulated integration efficiency likewise increase the experimentally observed expression levels. Demonstration of these tre

SUBMITTER: Marshall SS 

PROVIDER: S-EPMC5001948 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature