Mechanically Induced Chromatin Condensation Requires Cellular Contractility in Mesenchymal Stem Cells.

ABSTRACT: Mechanical cues play important roles in directing the lineage commitment of mesenchymal stem cells (MSCs). In this study, we explored the molecular mechanisms by which dynamic tensile loading (DL) regulates chromatin organization in this cell type. Our previous findings indicated that the application of DL elicited a rapid increase in chromatin condensation through purinergic signaling mediated by ATP. Here, we show that the rate and degree of condensation depends on the frequency and duration of mechanical loading, and that ATP release requires actomyosin-based cellular contractility. Increases in baseline cellular contractility via the addition of an activator of G-protein coupled receptors (lysophosphatidic acid) induced rapid ATP release, resulting in chromatin condensation independent

SUBMITTER: Heo SJ 

PROVIDER: S-EPMC5002070 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature