Human papillomavirus oncoprotein E6 upregulates c-Met through p53 downregulation.

ABSTRACT:

Purpose

Human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCC) carries a distinct clinical behaviour. c-Met oncogene is an important driver for tumour progression and its relationship with HPV in OPSCC was explored in the present study.

Experimental design

Knockdown of HPV oncogene E6 or p53 alone and in combination was performed to examine their effects on c-Met expression by Western blot and quantitative real-time polymerase chain reaction. The effects of c-Met inhibition on cell proliferation, migration, and colony formation were examined in HPV-positive head and neck squamous cell carcinoma (HNSCC) cells. Retrospectively collected OPSCC patient specimens (N = 78) were stained for c-Met by immunohistochemistry and the staining levels were corr

SUBMITTER: Qian G 

PROVIDER: S-EPMC5002257 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature