Validating silicon polytrodes with paired juxtacellular recordings: method and dataset.

ABSTRACT: Cross-validating new methods for recording neural activity is necessary to accurately interpret and compare the signals they measure. Here we describe a procedure for precisely aligning two probes for in vivo "paired-recordings" such that the spiking activity of a single neuron is monitored with both a dense extracellular silicon polytrode and a juxtacellular micropipette. Our new method allows for efficient, reliable, and automated guidance of both probes to the same neural structure with micrometer resolution. We also describe a new dataset of paired-recordings, which is available online. We propose that our novel targeting system, and ever expanding cross-validation dataset, will be vital to the development of new algorithms for automatically detecting/sorting single-units, characterizi

SUBMITTER: Neto JP 

PROVIDER: S-EPMC5002440 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature