An integrative approach for the large-scale identification of human genome kinases regulating cancer metastasis.

ABSTRACT: Kinases become one of important groups of drug targets. To identify more kinases being potential for cancer therapy, we developed an integrative approach for the large-scale screen of functional genes capable of regulating the main traits of cancer metastasis. We first employed self-assembled cell microarray to screen functional genes that regulate cancer cell migration using a human genome kinase siRNA library. We identified 81 genes capable of significantly regulating cancer cell migration. Following with invasion assays and bio-informatics analysis, we discovered that 16 genes with differentially expression in cancer samples can regulate both cell migration and invasion, among which 10 genes have been well known to play critical roles in the cancer development. The remaining 6 genes wer

SUBMITTER: Zhang H 

PROVIDER: S-EPMC5003042 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature