Rapid Expansion of Human Epithelial Stem Cells Suitable for Airway Tissue Engineering.

ABSTRACT:

Rationale

Stem cell-based tracheal replacement represents an emerging therapeutic option for patients with otherwise untreatable airway diseases including long-segment congenital tracheal stenosis and upper airway tumors. Clinical experience demonstrates that restoration of mucociliary clearance in the lungs after transplantation of tissue-engineered grafts is critical, with preclinical studies showing that seeding scaffolds with autologous mucosa improves regeneration. High epithelial cell-seeding densities are required in regenerative medicine, and existing techniques are inadequate to achieve coverage of clinically suitable grafts.

Objectives

To define a scalable cell culture system to deliver airway epithelium to clinical grafts.

Methods

Human respiratory epitheli

SUBMITTER: Butler CR 

PROVIDER: S-EPMC5003214 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature