Receptor Tyrosine Kinase Expression Predicts Response to Sunitinib in Breast Cancer.

ABSTRACT:

Background

Preliminary data indicate that tyrosine kinase inhibitors (TKIs) function through rearranged during transfection (RET) in breast cancer. However, TKIs are not specific and can block several receptor tyrosine kinases (RTKs). This study used cell lines and primary breast cancer specimens to determine factors associated with TKI response.

Methods

Proliferation was assessed after short interfering RNA knockdown with or without sunitinib in breast cancer cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). Breast cancer tissue and matched normal breast was obtained from 30 women with invasive breast carcinoma. Gene expression was assessed by reverse transcriptase-polymerase chain reaction. Fresh tissue was treated in vitro with sunitinib or

SUBMITTER: Spanheimer PM 

PROVIDER: S-EPMC5003404 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature