A newly identified mechanism involved in regulation of human mesenchymal stem cells by fibrous substrate stiffness.

ABSTRACT:

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Stiffness of biomaterial substrates plays a critical role in regulation of cell behavior. Although the effect of substrate stiffness on cell behavior has been extensively studied, molecular mechanisms of regulation rather than those involving cytoskeletal activities still remain elusive. In this study, we fabricated aligned ultrafine fibers and treated the fiber with different annealing temperatures to produce fibrous substrates with different stiffness. Human mesenchymal stem cells (hMSCs) were then cultured on these fibrous substrates. Our results showed that annealing treatment did not change the diameter of electrospun fibers but increased their polymer crystallinity and mechanical properties. The mRNA expression of RUNX2 was upregulated while the mRNA expression of

SUBMITTER: Yuan H 

PROVIDER: S-EPMC5003633 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature