Micro-computed tomography in murine models of cerebral cavernous malformations as a paradigm for brain disease.

ABSTRACT:

Background

Cerebral cavernous malformations (CCMs) are hemorrhagic brain lesions, where murine models allow major mechanistic discoveries, ushering genetic manipulations and preclinical assessment of therapies. Histology for lesion counting and morphometry is essential yet tedious and time consuming. We herein describe the application and validations of X-ray micro-computed tomography (micro-CT), a non-destructive technique allowing three-dimensional CCM lesion count and volumetric measurements, in transgenic murine brains.

New method

We hereby describe a new contrast soaking technique not previously applied to murine models of CCM disease. Volumetric segmentation and image processing paradigm allowed for histologic correlations and quantitative validations not previously re

SUBMITTER: Girard R 

PROVIDER: S-EPMC5003657 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature