Two-photon imaging of cerebral hemodynamics and neural activity in awake and anesthetized marmosets.

ABSTRACT:

Background

Marmosets are a powerful, emerging model for human behavior and neurological disorders. However, longitudinal imaging modalities that visualize both cellular structure and function within the cortex are not available in this animal model. Hence, we implemented an approach to quantify vascular topology, hemodynamics, and neural activity in awake marmosets using two-photon microscopy (2PM).

New method

Marmosets were acclimated to a custom stereotaxic system. AAV1-GCaMP5G was injected into somatosensory cortex to optically indicate neural activity, and a cranial chamber was implanted.

Results

Longitudinal 2PM revealed vasculature and neurons 500μm below the cortical surface. Vascular response and neural activity during sensory stimulation were preserved over 5

SUBMITTER: Santisakultarm TP 

PROVIDER: S-EPMC5003758 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature