Phosphocholine-containing ligands direct CRP induction of M2 macrophage polarization independent of T cell polarization: Implication for chronic inflammatory states.

ABSTRACT:

Introduction

We studied monocyte transendothelial migration and subsequent polarization into M1/M2 macrophages in response to C-reactive protein (CRP) with two disease-related ligands: (1) phosphocholine (PC) and (2) multilamellar liposomes containing both unoxidized and oxidized forms of the lipid, phosphatidylcholine. These ligands differ in biological origin: PC is present on bacterial cell walls while oxidized lipids are present in atherogenic lipids.

Methods

We used an in vitro model of human monocyte transendothelial migration and assessed the polarization of monocytes and T cells and signaling through Fcγ receptors in monocytes.

Results

CRP without ligands did not promote M2 macrophage differentiation over background levels. However, when paired with either lig

SUBMITTER: Trial J 

PROVIDER: S-EPMC5004283 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature