Nfib Promotes Metastasis through a Widespread Increase in Chromatin Accessibility.

ABSTRACT: Metastases are the main cause of cancer deaths, but the mechanisms underlying metastatic progression remain poorly understood. We isolated pure populations of cancer cells from primary tumors and metastases from a genetically engineered mouse model of human small cell lung cancer (SCLC) to investigate the mechanisms that drive the metastatic spread of this lethal cancer. Genome-wide characterization of chromatin accessibility revealed the opening of large numbers of distal regulatory elements across the genome during metastatic progression. These changes correlate with copy number amplification of the Nfib locus, and differentially accessible sites were highly enriched for Nfib transcription factor binding sites. Nfib is necessary and sufficient to increase chromatin accessibility at a lar

SUBMITTER: Denny SK 

PROVIDER: S-EPMC5004630 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature