Single-molecule RNA detection at depth by hybridization chain reaction and tissue hydrogel embedding and clearing.

ABSTRACT: Accurate and robust detection of mRNA molecules in thick tissue samples can reveal gene expression patterns in single cells within their native environment. Preserving spatial relationships while accessing the transcriptome of selected cells is a crucial feature for advancing many biological areas - from developmental biology to neuroscience. However, because of the high autofluorescence background of many tissue samples, it is difficult to detect single-molecule fluorescence in situ hybridization (smFISH) signals robustly in opaque thick samples. Here, we draw on principles from the emerging discipline of dynamic nucleic acid nanotechnology to develop a robust method for multi-color, multi-RNA imaging in deep tissues using single-molecule hybridization chain reaction (smHCR). Using this a

SUBMITTER: Shah S 

PROVIDER: S-EPMC5004914 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature