Hydrochlorothiazide Potentiates Contractile Activity of Mouse Cavernosal Smooth Muscle.

ABSTRACT:

Introduction

Hydrochlorothiazide has a negative influence on penile erection but little is known about the mechanism(s) involved.

Aims

To characterize the effects of this diuretic on mouse corpus cavernosum (CC) smooth muscle in vitro and ex vivo.

Methods

CC strips of C57BL/6 mice (12-16 weeks old) were mounted in organ baths containing Krebs-Henseleit solution and tissue reactivity was evaluated. Expression of genes encoding diuretic targets and enzymes involved in penile erection were evaluated by polymerase chain reaction.

Main outcome measures

Stimulation-response curves to phenylephrine (10 nmol/L-100 μmol/L) or to electrical field stimulation (1-32 Hz) were constructed, with or without hydrochlorothiazide. Strips of CC from mice after long-term hydrochlor

SUBMITTER: Gagliano-Juca T 

PROVIDER: S-EPMC5005312 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature