DNA Phosphorothioate Modification Plays a Role in Peroxides Resistance in Streptomyces lividans.

ABSTRACT: DNA phosphorothioation, conferred by dnd genes, was originally discovered in the soil-dwelling bacterium Streptomyces lividans, and thereafter found to exist in various bacterial genera. However, the physiological significance of this sulfur modification of the DNA backbone remains unknown in S. lividans. Our studies indicate that DNA phosphorothioation has a major role in resistance to oxidative stress in the strain. Although Streptomyces species express multiple catalase/peroxidase and organic hydroperoxide resistance genes to protect them against peroxide damage, a wild type strain of S. lividans exhibited two-fold to 10-fold higher survival, compared to a dnd (-) mutant, following treatment with peroxides. RNA-seq experiments revealed that, catalase and organic hydroperoxide resistance

SUBMITTER: Dai D 

PROVIDER: S-EPMC5005934 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature