In situ visualization of intracellular morphology of epidermal cells using stimulated Raman scattering microscopy.

ABSTRACT: Visualization of epidermal cells is important because the differentiation patterns of keratinocytes (KCs) are considered to be related to the functions and condition of skin. Optical microscopy has been widely used to investigate epidermal cells, but its applicability is still limited because of the need for sample fixation and staining. Here, we report our staining-free observation of epidermal cells in both tissue and culture by stimulated Raman scattering (SRS) microscopy that provides molecular vibrational contrast. SRS allowed us to observe a variety of cellular morphologies in skin tissue, including ladder-like structures in the spinous layer, enucleation of KCs in the granular layer, and three-dimensional cell column structures in the stratum corneum. We noticed that some cells in t

SUBMITTER: Egawa M 

PROVIDER: S-EPMC5005979 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature