Fully integrated reflection-mode photoacoustic, two-photon, and second harmonic generation microscopy in vivo.

ABSTRACT: The ability to obtain comprehensive structural and functional information from intact biological tissue in vivo is highly desirable for many important biomedical applications, including cancer and brain studies. Here, we developed a fully integrated multimodal microscopy that can provide photoacoustic (optical absorption), two-photon (fluorescence), and second harmonic generation (SHG) information from tissue in vivo, with intrinsically co-registered images. Moreover, using a delicately designed optical-acoustic coupling configuration, a high-frequency miniature ultrasonic transducer was integrated into a water-immersion optical objective, thus allowing all three imaging modalities to provide a high lateral resolution of ~290 nm with reflection-mode imaging capability, which is essential f

SUBMITTER: Song W 

PROVIDER: S-EPMC5006040 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature