Protein backbone ensemble generation explores the local structural space of unseen natural homologs.

ABSTRACT:

Motivation

Mutations in homologous proteins affect changes in the backbone conformation that involve a complex interplay of forces which are difficult to predict. Protein design algorithms need to anticipate these backbone changes in order to accurately calculate the energy of the structure given an amino acid sequence, without knowledge of the final, designed sequence. This is related to the problem of predicting small changes in the backbone between highly similar sequences.

Results

We explored the ability of the Rosetta suite of protein design tools to move the backbone from its position in one structure (template) to its position in a close homologous structure (target) as a function of the diversity of a backbone ensemble constructed using the template structure, the pe

SUBMITTER: Schenkelberg CD 

PROVIDER: S-EPMC5006151 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature