Biomimetic surface patterning for long-term transmembrane access.

ABSTRACT: Here we present a planar patch clamp chip based on biomimetic cell membrane fusion. This architecture uses nanometer length-scale surface patterning to replicate the structure and function of membrane proteins, creating a gigaohm seal between the cell and a planar electrode array. The seal is generated passively during cell spreading, without the application of a vacuum to the cell surface. This interface can enable cell-attached and whole-cell recordings that are stable to 72 hours, and generates no visible damage to the cell. The electrodes can be very small (<5 μm) and closely packed, offering a high density platform for cellular measurement.

SUBMITTER: VanDersarl JJ 

PROVIDER: S-EPMC5006161 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature