Infectivity of Immature Neurons to Zika Virus: A Link to Congenital Zika Syndrome.

ABSTRACT:

Background

Epidemiological data strongly suggest that microcephaly cases in Brazil are associated with the ongoing epidemic of Zika virus (ZIKV). In order to further solidify the possible link, we investigated the infectivity of ZIKV using various neuroblastoma (NB) cell lines.

Methods

Six undifferentiated, two terminally differentiated and two retinoic acid (RA) -induced, partially differentiated cell lines were exposed to ZIKV strain PRVABC59, which is genetically similar to the French Polynesia strain, with 97-100% genetic homology to the current ZIKV strain found in Brazil. All infections were confirmed by real-time PCR (RT-qPCR), immunofluorescence assay (IFA) probing with anti-flavivirus E antibody, and evaluation of cytopathic effects.

Findings

ZIKV infected al

SUBMITTER: Hughes BW 

PROVIDER: S-EPMC5006602 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature