Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices.

ABSTRACT: Organs-on-a-chip has emerged as a powerful tool for pharmacological and physiological studies. A key part in the construction of such a model is the ability to pattern or culture cells in a biomimetic fashion. Most of the reported cells-on-a-chip models integrate cells on a flat surface, which does not accurately represent the extracellular matrix that they experience in vivo. Electrospinning, a technique used to generate sub-micron diameter polymer fibers, has been used as an in vitro cell culture substrate and for tissue engineering applications. Electrospinning of fibers directly into a fully sealed fluidic channel using a conventional setup has not been possible due to issues of confining the fibers into a discrete network. In this work, a dynamic focusing method was developed, with th

SUBMITTER: Chen C 

PROVIDER: S-EPMC5007176 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature