Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth.

ABSTRACT: Dynamic control of gene expression can have far-reaching implications for biotechnological applications and biological discovery. Thanks to the advantages of light, optogenetics has emerged as an ideal technology for this task. Current state-of-the-art methods for optical expression control fail to combine precision with repeatability and cannot withstand changing operating culture conditions. Here, we present a novel fully automatic experimental platform for the robust and precise long-term optogenetic regulation of protein production in liquid Escherichia coli cultures. Using a computer-controlled light-responsive two-component system, we accurately track prescribed dynamic green fluorescent protein expression profiles through the application of feedback control, and show that the system

SUBMITTER: Milias-Argeitis A 

PROVIDER: S-EPMC5007438 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature