Stand-Sit Microchip for High-Throughput, Multiplexed Analysis of Single Cancer Cells.

ABSTRACT: Cellular heterogeneity in function and response to therapeutics has been a major challenge in cancer treatment. The complex nature of tumor systems calls for the development of advanced multiplexed single-cell tools that can address the heterogeneity issue. However, to date such tools are only available in a laboratory setting and don't have the portability to meet the needs in point-of-care cancer diagnostics. Towards that application, we have developed a portable single-cell system that is comprised of a microchip and an adjustable clamp, so on-chip operation only needs pipetting and adjusting of clamping force. Up to 10 proteins can be quantitated from each cell with hundreds of single-cell assays performed in parallel from one chip operation. We validated the technology and analyzed th

SUBMITTER: Ramirez L 

PROVIDER: S-EPMC5007481 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature