Differential regulation of the histone chaperone HIRA during muscle cell differentiation by a phosphorylation switch.

ABSTRACT: Replication-independent incorporation of variant histone H3.3 has a profound impact on chromatin function and numerous cellular processes, including the differentiation of muscle cells. The histone chaperone HIRA and H3.3 have essential roles in MyoD regulation during myoblast differentiation. However, the precise mechanism that determines the onset of H3.3 deposition in response to differentiation signals is unclear. Here we show that HIRA is phosphorylated by Akt kinase, an important signaling modulator in muscle cells. By generating a phosphospecific antibody, we found that a significant amount of HIRA was phosphorylated in myoblasts. The phosphorylation level of HIRA and the occupancy of phosphorylated protein on muscle genes gradually decreased during cellular differentiation. Remarka

SUBMITTER: Yang JH 

PROVIDER: S-EPMC5007640 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature