Erythropoietin (EPO)-receptor signaling induces cell death of primary myeloma cells in vitro.

ABSTRACT:

Background

Multiple myeloma is an incurable complex disease characterized by clonal proliferation of malignant plasma cells in a hypoxic bone marrow environment. Hypoxia-dependent erythropoietin (EPO)-receptor (EPOR) signaling is central in various cancers, but the relevance of EPOR signaling in multiple myeloma cells has not yet been thoroughly investigated.

Methods

Myeloma cell lines and malignant plasma cells isolated from bone marrow of myeloma patients were used in this study. Transcript levels were analysed by quantitative PCR and cell surface levels of EPOR in primary cells by flow cytometry. Knockdown of EPOR by short interfering RNA was used to show specific EPOR signaling in the myeloma cell line INA-6. Flow cytometry was used to assess viability in primary cells t

SUBMITTER: Vatsveen TK 

PROVIDER: S-EPMC5007700 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature