Identification of a Halogenase Involved in the Biosynthesis of Ochratoxin A in Aspergillus carbonarius.

ABSTRACT:

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Aspergillus carbonarius is the main responsible fungus of ochratoxin A (OTA) contamination of grapes and derived products. To date, the biosynthetic mechanism of this mycotoxin has been partially elucidated. Availability of genome sequence of A. carbonarius has allowed the identification of a putative gene cluster involved in OTA biosynthesis. This region hosts the previously characterized AcOTAnrps and AcOTApks genes encoding two key enzymes of the biosynthetic pathway. At about 4,400 nucleotides downstream of these loci, a gene encoding a putative flavin dependent-halogenase came out from the annotation data. Its proximity to OTA biosynthetic genes and its sequence analysis have suggested a role in the biosynthesis of OTA, directed to the introduction of the chlorine a

SUBMITTER: Ferrara M 

PROVIDER: S-EPMC5007760 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature