High Level of Spinosad Production in the Heterologous Host Saccharopolyspora erythraea.

ABSTRACT:

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Spinosad, a highly effective insecticide, has an excellent environmental and mammalian toxicological profile. Global market demand for spinosad is huge and growing. However, after much effort, there has been almost no improvement in the spinosad yield from the original producer, Saccharopolyspora spinosa Here, we report the heterologous expression of spinosad using Saccharopolyspora erythraea as a host. The native erythromycin polyketide synthase (PKS) genes in S. erythraea were replaced by the assembled spinosad gene cluster through iterative recombination. The production of spinosad could be detected in the recombinant strains containing the whole biosynthesis gene cluster. Both metabolic engineering and UV mutagenesis were applied to further improve the yield of spino

SUBMITTER: Huang J 

PROVIDER: S-EPMC5007771 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature