Enrichment of Root Endophytic Bacteria from Populus deltoides and Single-Cell-Genomics Analysis.

ABSTRACT:

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Bacterial endophytes that colonize Populus trees contribute to nutrient acquisition, prime immunity responses, and directly or indirectly increase both above- and below-ground biomasses. Endophytes are embedded within plant material, so physical separation and isolation are difficult tasks. Application of culture-independent methods, such as metagenome or bacterial transcriptome sequencing, has been limited due to the predominance of DNA from the plant biomass. Here, we describe a modified differential and density gradient centrifugation-based protocol for the separation of endophytic bacteria from Populus roots. This protocol achieved substantial reduction in contaminating plant DNA, allowed enrichment of endophytic bacteria away from the plant material, and enabled sin

SUBMITTER: Utturkar SM 

PROVIDER: S-EPMC5007785 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature