A drug-compatible and temperature-controlled microfluidic device for live-cell imaging.

ABSTRACT: Monitoring cellular responses to changes in growth conditions and perturbation of targeted pathways is integral to the investigation of biological processes. However, manipulating cells and their environment during live-cell-imaging experiments still represents a major challenge. While the coupling of microfluidics with microscopy has emerged as a powerful solution to this problem, this approach remains severely underexploited. Indeed, most microdevices rely on the polymer polydimethylsiloxane (PDMS), which strongly absorbs a variety of molecules commonly used in cell biology. This effect of the microsystems on the cellular environment hampers our capacity to accurately modulate the composition of the medium and the concentration of specific compounds within the microchips, with implicatio

SUBMITTER: Chen T 

PROVIDER: S-EPMC5008015 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature