Ontology highlight
ABSTRACT:
SUBMITTER: Ciampi O
PROVIDER: S-EPMC5009184 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Stem cell research 20160603 1
Generating human podocytes in vitro could offer a unique opportunity to study human diseases. Here, we describe a simple and efficient protocol for obtaining functional podocytes in vitro from human induced pluripotent stem cells. Cells were exposed to a three-step protocol, which induced their differentiation into intermediate mesoderm, then into nephron progenitors and, finally, into mature podocytes. After differentiation, cells expressed the main podocyte markers, such as synaptopodin, WT1, ...[more]