Replication Timing of Human Telomeres is Conserved during Immortalization and Influenced by Respective Subtelomeres.

ABSTRACT: Telomeres are specific structures that protect chromosome ends and act as a biological clock, preventing normal cells from replicating indefinitely. Mammalian telomeres are replicated throughout S-phase in a predetermined order. However, the mechanism of this regulation is still unknown. We wished to investigate this phenomenon under physiological conditions in a changing environment, such as the immortalization process to better understand the mechanism for its control. We thus examined the timing of human telomere replication in normal and SV40 immortalized cells, which are cytogenetically very similar to cancer cells. We found that the timing of telomere replication was globally conserved under different conditions during the immortalization process. The timing of telomere replication w

SUBMITTER: Piqueret-Stephan L 

PROVIDER: S-EPMC5009427 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature