Scleroderma dermal microvascular endothelial cells exhibit defective response to pro-angiogenic chemokines.

ABSTRACT:

Objectives

Angiogenesis plays a critical role in SSc (scleroderma). The aim of this study was to examine the expression of growth-regulated protein-γ (Gro-γ/CXCL3), granulocyte chemotactic protein 2 (GCP-2/CXCL6) and their receptor CXCR2 in endothelial cells (ECs) isolated from SSc skin and determine whether these cells mount an angiogenic response towards pro-angiogenic chemokines. The downstream signalling pathways as well as the pro-angiogenic transcription factor inhibitor of DNA-binding protein 1 (Id-1) were also examined.

Methods

Skin biopsies were obtained from patients with dcSSc. ECs were isolated via magnetic positive selection. Angiogenesis was measured by EC chemotaxis assay.

Results

Gro-γ/CXCL3 and GCP-2/CXCL6 were minimally expressed in both skin types b

SUBMITTER: Tsou PS 

PROVIDER: S-EPMC5009475 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature