The green ash transcriptome and identification of genes responding to abiotic and biotic stresses.

ABSTRACT:

Background

To develop a set of transcriptome sequences to support research on environmental stress responses in green ash (Fraxinus pennsylvanica), we undertook deep RNA sequencing of green ash tissues under various stress treatments. The treatments, including emerald ash borer (EAB) feeding, heat, drought, cold and ozone, were selected to mimic the increasing threats of climate change and invasive pests faced by green ash across its native habitat.

Results

We report the generation and assembly of RNA sequences from 55 green ash samples into 107,611 putative unique transcripts (PUTs). 52,899 open reading frames were identified. Functional annotation of the PUTs by comparison to the Uniprot protein database identified matches for 63 % of transcripts and for 98 % of transcript

SUBMITTER: Lane T 

PROVIDER: S-EPMC5009568 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature