Screening for Chemical Toxicity Using Cryopreserved Precision Cut Lung Slices.

ABSTRACT: To assess chemical toxicity, current high throughput screening (HTS) assays rely primarily on in vitro measurements using cultured cells. Responses frequently differ from in vivo results due to the lack of physical and humoral interactions provided by the extracellular matrix, cell-cell interactions, and other molecular components of the native organ. To more accurately reproduce organ complexity in HTS, we developed an organotypic assay using the cryopreserved precision cut lung slice (PCLS) from rats and mice. Compared to the never-frozen PCLS, their frozen-thawed counterpart slices showed viability or metabolic activity that is decreased to an extent comparable to that observed in other cryopreserved cells and tissues, but shows no differences in further changes in cell viability, mitoc

SUBMITTER: Watson CY 

PROVIDER: S-EPMC5009619 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature