MicrobeJ, a tool for high throughput bacterial cell detection and quantitative analysis.

ABSTRACT: Single-cell analysis of bacteria and subcellular protein localization dynamics has shown that bacteria have elaborate life cycles, cytoskeletal protein networks and complex signal transduction pathways driven by localized proteins. The volume of multidimensional images generated in such experiments and the computation time required to detect, associate and track cells and subcellular features pose considerable challenges, especially for high-throughput experiments. There is therefore a need for a versatile, computationally efficient image analysis tool capable of extracting the desired relationships from images in a meaningful and unbiased way. Here, we present MicrobeJ, a plug-in for the open-source platform ImageJ(1). MicrobeJ provides a comprehensive framework to process images derived

SUBMITTER: Ducret A 

PROVIDER: S-EPMC5010025 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature