Structural Determinants of Sleeping Beauty Transposase Activity.

ABSTRACT: Transposases are important tools in genome engineering, and there is considerable interest in engineering more efficient ones. Here, we seek to understand the factors determining their activity using the Sleeping Beauty transposase. Recent work suggests that protein coevolutionary information can be used to classify groups of physically connected, coevolving residues into elements called "sectors", which have proven useful for understanding the folding, allosteric interactions, and enzymatic activity of proteins. Using extensive mutagenesis data, protein modeling and analysis of folding energies, we show that (i) The Sleeping Beauty transposase contains two sectors, which span across conserved domains, and are enriched in DNA-binding residues, indicating that the DNA binding and endonuclea

SUBMITTER: Abrusan G 

PROVIDER: S-EPMC5010145 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature