Thermally-assisted ultrasonic separation of giant vesicles.

ABSTRACT: We report on a newly-developed membrane stiffness-based separation of vesicles using a thermally-assisted acoustophoretic approach. By tuning the temperature, we achieved the separation of vesicles of the same size, shape, and charge but with different stiffness values. It was observed that at a specific transition point, the acoustic contrast factor of vesicles changed sign from positive to negative. This change was mainly due to the change in the acoustic compressibility of the vesicles, which is inversely proportional to stiffness. The acoustic contrast temperature, corresponding to the temperature at which the acoustic contrast factor switches sign, was determined to be unique to the composition of the vesicles. This unique temperature signature allowed us to develop a separation metho

SUBMITTER: Dolatmoradi A 

PROVIDER: S-EPMC5010174 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature