Development and molecular characterization of a doubled haploid population derived from a hybrid between japonica rice and wide compatible indica rice.

ABSTRACT: Doubled haploid (DH) populations, particularly those from subspecies crosses possessing the wide compatible gene S5ⁿ , are important germplasm resources for rice genetic studies and breeding, but their feature and potential have not been fully assessed and explored. In the present study, we produced a DH population from the hybrid of japonica 668B and wide compatible indica T23. Genotyping of the S5 locus with allele-specific markers for ORF3, ORF4 and ORF5 revealed a potential recombination hot spot in the ORF3-ORF4 region. Haplotyping analysis revealed that 21/34 subspecies specific Indel markers segregated in distortion in the DH population, with a few lines having indica alleles either extremely low (1.7%)

SUBMITTER: Nguyen H 

PROVIDER: S-EPMC5010298 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature