Persistence and evolution of allergen-specific IgE repertoires during subcutaneous specific immunotherapy.

ABSTRACT:

Background

Specific immunotherapy (SIT) is the only treatment with proved long-term curative potential in patients with allergic disease. Allergen-specific IgE is the causative agent of allergic disease, and antibodies contribute to SIT, but the effects of SIT on aeroallergen-specific B-cell repertoires are not well understood.

Objective

We sought to characterize the IgE sequences expressed by allergen-specific B cells and track the fate of these B-cell clones during SIT.

Methods

We used high-throughput antibody gene sequencing and identification of allergen-specific IgE with combinatorial antibody fragment library technology to analyze immunoglobulin repertoires of blood and the nasal mucosa from aeroallergen-sensitized subjects before and during the first year of su

SUBMITTER: Levin M 

PROVIDER: S-EPMC5010428 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature