Purification and characterization of laccase from Marasmius species BBKAV79 and effective decolorization of selected textile dyes.

ABSTRACT: A novel laccase-producing white-rot fungus, Marasmius sp. BBKAV79 (Genbank Accession Number-KP455496, KP455497), was isolated and subjected to purification, characterization and dye decolorization study. The purified enzyme was obtained with a specific activity of 0.226 U mg^-1 protein and a final yield of 13.5 %. The enzyme was found to be a monomeric protein with a molecular mass of ~75 kDa as estimated by non-denaturing polyacrylamide gel electrophoresis (PAGE) and further confirmed with zymogram analysis. The optimal pH and temperature of the laccase was recorded to be 5.5 and 40 °C, respectively. The metal ions Hg²⁺ and Ag⁺ were found to drastically inhibit the activity of laccase at the rate of 96.6 and 96.5 %, respectively. Nevertheless, Fe³⁺

SUBMITTER: Vantamuri AB 

PROVIDER: S-EPMC5010537 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature