A validated cellular biobank for β-thalassemia.

ABSTRACT:

Background

Cellular biobanking is a key resource for collaborative networks planning to use same cells in studies aimed at solving a variety of biological and biomedical issues. This approach is of great importance in studies on β-thalassemia, since the recruitment of patients and collection of specimens can represent a crucial and often limiting factor in the experimental planning.

Methods

Erythroid precursor cells were obtained from 72 patients, mostly β-thalassemic, expanded and cryopreserved. Expression of globin genes was analyzed by real time RT-qPCR. Hemoglobin production was studied by HPLC.

Results

In this paper we describe the production and validation of a Thal-Biobank constituted by expanded erythroid precursor cells from β-thalassemia patients. The bioban

SUBMITTER: Cosenza LC 

PROVIDER: S-EPMC5010737 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature