Fluorescence In situ Hybridization: Cell-Based Genetic Diagnostic and Research Applications.

ABSTRACT: Fluorescence in situ hybridization (FISH) is a macromolecule recognition technology based on the complementary nature of DNA or DNA/RNA double strands. Selected DNA strands incorporated with fluorophore-coupled nucleotides can be used as probes to hybridize onto the complementary sequences in tested cells and tissues and then visualized through a fluorescence microscope or an imaging system. This technology was initially developed as a physical mapping tool to delineate genes within chromosomes. Its high analytical resolution to a single gene level and high sensitivity and specificity enabled an immediate application for genetic diagnosis of constitutional common aneuploidies, microdeletion/microduplication syndromes, and subtelomeric rearrangements. FISH tests using panels of gene-specifi

SUBMITTER: Cui C 

PROVIDER: S-EPMC5011256 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature