Troglitazone Enhances the Apoptotic Response of DLD-1 Colon Cancer Cells to Photodynamic Therapy.

ABSTRACT:

Purpose

The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells.

Materials and methods

The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses.

Results

Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent

SUBMITTER: Park H 

PROVIDER: S-EPMC5011284 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature