Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells.

ABSTRACT:

Objective

Micro-RNAs (miRNAs) are a class of posttranscriptional regulators that play crucial roles in various biological processes. Emerging evidence suggests a direct link between miRNAs and development of several diseases including type 2 diabetes (T2D). In this study, we aimed to investigate the effect of predicted miRNA and target genes on insulin resistance.

Materials and methods

This experimental study was conducted on the C2C12 cell line. Using bioinformatics tools miRNA-135 and two respective target genes-insulin receptor (Insr) and vesicle associated membrane protein 2 (Vamp2)were selected as potential factors involved in insulin resistance process. Levels of glucose uptake miRNA expression and respective gene targets were determined after cell transfaction by miR-

SUBMITTER: Honardoost M 

PROVIDER: S-EPMC5011323 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature