Canine Infections and Partial S Segment Sequence Analysis of Toscana Virus in Turkey.

ABSTRACT:

Introduction

Toscana virus (TOSV) is a sandfly-borne bunyavirus with a significant public health impact. Preliminary studies have revealed TOSV exposure in dogs and they were suggested as potential reservoirs. This study was performed to characterize canine TOSV infections in an endemic region. Sequencing of TOSV small (S) segment in several previously identified specimens was also undertaken to reveal viral genealogy.

Materials and methods

Canine and feline plasma were collected in several districts of Mersin province, Mediterranean Anatolia, Turkey, during May-September, 2015. Phlebovirus RNA was screened through two nested polymerase chain reaction (PCR) assays, targeting S and large (L) segments of the viral genome. A kinetoplast minicircle nested PCR was employed for Le

SUBMITTER: Dincer E 

PROVIDER: S-EPMC5011618 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature