Food-induced changes of lipids in rat neuronal tissue visualized by ToF-SIMS imaging.

ABSTRACT: Time of flight secondary ion mass spectrometry (ToF-SIMS) was used to image the lipid localization in brain tissue sections from rats fed specially processed cereals (SPC). An IonTof 5 instrument equipped with a Bi cluster ion gun was used to analyze the tissue sections. Data from 15 brain samples from control and cereal-fed rats were recorded and exported to principal components analysis (PCA). The data clearly show changes of certain lipids in the brain following cereal feeding. PCA score plots show a good separation in lipid distribution between the control and the SPC-fed group. The loadings plot reveal that the groups separated mainly due to changes in cholesterol, vitamin E and c18:2, c16:0 fatty acid distribution as well as some short chain monocarboxylic fatty acid compositions. Th

SUBMITTER: Dowlatshahi Pour M 

PROVIDER: S-EPMC5011716 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature