Molecular mechanism leading to SAHA-induced autophagy in tumor cells: evidence for a p53-dependent pathway.

ABSTRACT:

Background

Recent studies indicated that histone deacetylase inhibitors (HDACi), a class of anticancer agents, are in addition to their ability of apoptosis induction also capable of provoking autophagy. Promoted by the treatment of malignant uterine sarcoma cells with the HDACi suberoylanilide hydroxamic acid (SAHA), we previously demonstrated predominant dose-dependent activation of autophagy in ESS-1 cells, but prevalent induction of apoptosis in MES-SA cells.

Methods

In order to extend our previous studies, SAHA-treated ESS-1 and MES-SA cells were monitored for protein expression to reveal differences in known markers of apoptosis explaining the different cytotoxic responses. Further analysis of the identified candidate protein included cell rescue experiments by gene tr

SUBMITTER: Frohlich LF 

PROVIDER: S-EPMC5011867 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature