Rapid induction of GFP expression by the nitrate reductase promoter in the diatom Phaeodactylum tricornutum.

ABSTRACT: An essential prerequisite for a controlled transgene expression is the choice of a suitable promoter. In the model diatom Phaeodactylum tricornutum, the most commonly used promoters for trans-gene expression are the light dependent lhcf1 promoters (derived from two endogenous genes encoding fucoxanthin chlorophyll a/c binding proteins) and the nitrate dependent nr promoter (derived from the endogenous nitrate reductase gene). In this study, we investigated the time dependent expression of the green fluorescent protein (GFP) reporter under control of the nitrate reductase promoter in independently genetically transformed P. tricornutum cell lines following induction of expression by change of the nitrogen source in the medium via flow cytometry, microscopy and western blotting. In all inves

SUBMITTER: Chu L 

PROVIDER: S-EPMC5012323 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature