A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer.

ABSTRACT:

Background & aims

The lack of a preclinical model of progressive non-alcoholic steatohepatitis (NASH) that recapitulates human disease is a barrier to therapeutic development.

Methods

A stable isogenic cross between C57BL/6J (B6) and 129S1/SvImJ (S129) mice were fed a high fat diet with ad libitum consumption of glucose and fructose in physiologically relevant concentrations and compared to mice fed a chow diet and also to both parent strains.

Results

Following initiation of the obesogenic diet, B6/129 mice developed obesity, insulin resistance, hypertriglyceridemia and increased LDL-cholesterol. They sequentially also developed steatosis (4-8weeks), steatohepatitis (16-24weeks), progressive fibrosis (16weeks onwards) and spontaneous hepatocellular cancer (HCC). There

SUBMITTER: Asgharpour A 

PROVIDER: S-EPMC5012902 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature