Alcohol-induced suppression of KDM6B dysregulates the mineralization potential in dental pulp stem cells.

ABSTRACT: Epigenetic changes, such as alteration of DNA methylation patterns, have been proposed as a molecular mechanism underlying the effect of alcohol on the maintenance of adult stem cells. We have performed genome-wide gene expression microarray and DNA methylome analysis to identify molecular alterations via DNA methylation changes associated with exposure of human dental pulp stem cells (DPSCs) to ethanol (EtOH). By combined analysis of the gene expression and DNA methylation, we have found a significant number of genes that are potentially regulated by EtOH-induced DNA methylation. As a focused approach, we have also performed a pathway-focused RT-PCR array analysis to examine potential molecular effects of EtOH on genes involved in epigenetic chromatin modification enzymes, fibroblastic ma

SUBMITTER: Hoang M 

PROVIDER: S-EPMC5012956 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature